Chapter 8: Introduction to constraint-based modelling #

A protocol for generating a high-quality genome-scale metabolic reconstruction
Thiele and Palsson (2010) Nature Protocols

The classic paper discussing a detailed 96-step protocol for careful manual curation and reconstruction of a constraint-based model.

What is flux balance analysis?
Orth et al. (2010) Nature Biotechnology

A classic pedagogical piece introducing flux balance analysis.

Advances in flux balance analysis
Kauffman et al. (2003) Current Opinion in Biotechnology

The classic review on FBA.

Flux balance analysis of biological systems: applications and challenges
Raman and Chandra (2009) Briefings in Bioinformatics

A simple review on constraint-based modelling approaches, with an illustration of a simple FBA.